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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-500mL
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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-500mL
A 10X Concentrated solution of Phosphate Buffered Saline with Tween 20. PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays.
PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-500mL
Item #: 83467411

PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-500mL

Item #: 83467411

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A 10X Concentrated solution of Phosphate Buffered Saline with Tween 20. PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays.
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What Stands Out

Versatile Solution
This PBST solution is ideal for various laboratory applications, including immunoassays and protein analysis, catering to a wide range of scientific needs.
Optimized Formula
The precise formulation ensures consistent performance and reliability in experiments, minimizing variability and enhancing reproducibility for researchers.
Convenient Packaging
Available in a 500mL size, this ready-to-use solution saves time in preparation and reduces contamination risks, providing a hassle-free option for laboratory workflows.

Product Details

Shop PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-500mL online at a best price in Sierra Leone. B09V5LQSKN
Container TypeBottle
Item FormLiquid
Item Weight16 ounces (453.6 grams)
Volume1 Liters
Brand NameeCon Lab Supply
ManufacturerCiro Mfg
Part Number23.105.12
Included ComponentsBottle
BrandeCon Lab Supply
Item Volume1 Liters
Package InformationBottle

Who Should Buy?

Suitable For
  • Lab Researchers

    Ideal for laboratory settings where buffer solutions are essential for experiments involving protein interactions and immunoassays.

  • Biotechnology Applications

    Beneficial for biotech firms utilizing PBST in the production of recombinant proteins and assay development.

  • Diagnostic Laboratories

    Useful in clinical settings for washing steps in ELISA tests and other diagnostic methods, ensuring proper assay conditions.

Not Suitable For
  • Home Use

    Not suitable for casual or home experiments due to need for precise measurements and sterile conditions.

Product Description

PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-500mL

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Customer Questions & Answers

  • Question: What is PBST [10X] used for?

    Answer: PBST [10X] is primarily used as a wash buffer in various biological and biochemical applications, particularly in immunoassays and molecular biology techniques. It is designed to maintain a stable pH and ionic strength while also adding detergent (Tween-20) to minimize non-specific interactions. This makes it ideal for applications like ELISA, western blotting, and cell culture, where a clean background is crucial for results.
  • Question: What components are in PBST [10X]?

    Answer: The composition of PBST [10X] includes 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, and 0.5% Tween-20, with a pH of 7.4. Each component plays a vital role: phosphates provide buffering capacity, NaCl maintains osmotic balance, and Tween-20 reduces non-specific binding during assays. Understanding these components can help in adjusting the buffer for specific experimental conditions.
  • Question: How is PBST [10X] diluted for use?

    Answer: To use PBST [10X], it's typically diluted to a working concentration, commonly to 1X. This involves mixing one part of the 10X solution with nine parts of distilled or deionized water. For example, to prepare 1 liter of 1X PBST, you would combine 100 mL of the 10X solution with 900 mL of water. Proper dilution ensures optimal performance in experimental protocols.
  • Question: Why is Tween-20 included in PBST [10X]?

    Answer: Tween-20 is included in PBST [10X] as a non-ionic detergent that reduces non-specific binding in assays. It helps to minimize background noise by blocking sites that might otherwise capture unwanted proteins or antibodies. This is crucial when high specificity is required in techniques like ELISA or immunohistochemistry, allowing for clearer and more accurate results.
  • Question: Can PBST [10X] be used for cell culture?

    Answer: Yes, PBST [10X] can be utilized in cell culture, primarily during washing steps or to maintain pH stability in media. However, it is essential to ensure that the concentration of Tween-20 is relevant to the cell type being used, as some cells can be sensitive to detergents. It's often used for washing adherent cells in assays where precise protein interactions are investigated.
  • Question: Is PBST [10X] safe to use in laboratory settings?

    Answer: PBST [10X] is generally considered safe for laboratory environments, as its components are commonly used in biological research. However, like all chemical reagents, it should be handled with care. Always use appropriate personal protective equipment (PPE), work in a well-ventilated area, and follow your laboratory's safety protocols to ensure a safe working environment.
  • Question: How long can I store PBST [10X]?

    Answer: PBST [10X] can typically be stored for several months when properly sealed and kept at a stable temperature, away from direct light. Over time, components may degrade, affecting buffer performance. It's advisable to label the container with the preparation date and inspect the solution before use to ensure clarity and absence of precipitates, indicating that it remains effective for your work.
  • Question: What pH should PBST [10X] be adjusted to?

    Answer: The optimal pH for PBST [10X] is 7.4, which is physiological and suitable for most biological assays. This pH level ensures that biological molecules remain stable and functional during experimentation. If needed, pH can be adjusted using small amounts of hydrochloric acid or sodium hydroxide, but always verify the pH after adjustments to ensure compatibility with your specific applications.
  • Question: How do I dispose of unused PBST [10X]?

    Answer: Disposal of unused PBST [10X] should adhere to your institution's hazardous waste guidelines. Generally, it's classified as non-hazardous, but it's crucial to check if any specific regulations apply due to the Tween-20 component. If unsure, consult your laboratory's safety officer or environmental health and safety guidelines for proper disposal methods to minimize environmental impact.
  • Question: Where can I buy PBST [10X] in Sierra Leone?

    Answer: You can purchase PBST [10X] from Ubuy in Sierra Leone. Ubuy offers a wide range of laboratory reagents and buffers, including PBST [10X]. Their platform is tailored for buyers looking for quality scientific products and provides access to various suppliers, ensuring you can find the product you need easily.

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Features & Benefits

  • Comprehensive range of biological buffers for research, diagnostics, drug development, and manufacturing
  • Manufactured in an ISO 9001 and 13485 certified facility in the USA
  • 10X Concentrated solution of Phosphate Buffered Saline with Tween 20, ideal for Western blot membranes and ELISA assays
  • Each production lot manufactured in stringent Good Manufacturing Practices and quality control tests
  • Optimal formulation designed to effectively remove excess material without disrupting the antigen/antibody binding reaction

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