IBI Scientific
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IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations
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IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations
IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations
Item #: 1290416

IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations

82% of respondents would recommend this to a friend

Item #: 1290416

SLE 1293

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What Stands Out

Comprehensive Kit
Includes all necessary reagents for efficient and reliable DNA fragment extraction, ensuring high yield and purity for your samples, ideal for research and diagnostic applications.
User-Friendly
Designed for simplicity, the protocol allows for quick and easy processing, making it accessible for researchers of all skill levels, reducing the time spent on complex procedures.
Cost-Effective
Provides four extractions per kit, delivering excellent value for money, particularly for laboratories or institutions on a budget without compromising on performance.

Product Details

Shop IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations online at a best price in Sierra Leone. B0074NQKKA
  • Kit includes (4) DF columns, (4) 2mL collection tubes, 3mL DF buffer, 1mL wash buffer (4mL add ethanol) and 1mL elution buffer
  • For DNA fragments (100 bp-10 Kb) from agarose gel, PCR, or other enzymatic reactions
  • Spin column format with 10 microgram DNA binding capacity
  • Expectant yield is 80-90 percent for gel extraction and 90-95 percent for PCR clean-up
  • Applications include PCR fluorescent or radioactive sequencing, restriction digestion, DNA labeling, ligation
  • Operation time: 15 min for PCR clean-up, 20 min for gel extraction, with 20-50 microliter elution volume

Who Should Buy?

Suitable For
  • Molecular Biologists

    Ideal for molecular biologists who require efficient DNA purification for analysis and cloning in research settings.

  • Students

    Suitable for students conducting experiments in genetics or microbiology labs needing reliable DNA extraction kits.

  • Small Laboratories

    Perfect for small labs needing cost-effective solutions for DNA fragment extraction without compromising quality.

Not Suitable For
  • High-Volume Users

    Not suitable for facilities requiring high-throughput DNA extraction due to limited sample preparation capacity.

Product Description

IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations

About This Item

Introducing the IBI Scientific IB47010 GelPCR DNA Fragment Extraction Sample Kit for 4 Preparations. This kit is a must-have tool for anyone working with agarose gel, PCR, or other enzymatic reactions. With the Gel/PCR DNA Fragments Extraction Kit, you can easily recover or concentrate DNA fragments ranging from 100 bp to 10 Kb.

The unique design of this kit ensures efficient extraction and purification of the DNA fragments without the need for phenol extraction or alcohol precipitation. Using chaotropic salt, the Gel/PCR DNA Fragments Extraction Kit dissolves agarose gel and denatures enzymes, allowing the DNA fragments to be bound by the glass fiber matrix of the spin column. Contaminants are easily removed with the Wash Buffer, which contains ethanol, while the purified DNA fragments are eluted using a low salt Elution Buffer or TE. This comprehensive kit is not only time-efficient, with the entire procedure taking only 20 minutes, but it also provides excellent recovery rates - up to 90 percent for Gel Extraction and up to 95 percent for PCR Cleanup. Once purified, the DNA fragments are ready for immediate use in a variety of applications, including PCR, fluorescent or radioactive sequencing, restriction enzyme digestion, DNA labeling, and ligation. What sets the Gel/PCR DNA Fragments Extraction Kit apart is its ability to deliver high recoveries for both small base pair DNA fragments (50-200 bp) and large base pair DNA fragments (greater than 8 Kb).

Each batch of the Gel/PCR DNA Fragments Extraction Kit is tested extensively for quality assurance, ensuring reliable performance and consistent results. Please note that the DF buffer in this kit contains guanidine thiocyanate, which is a harmful irritant. It is important to always wear a lab coat, disposable gloves, and protective goggles when using this kit to ensure your safety. If you are looking for a reliable and efficient solution for your DNA fragment extraction needs, look no further than the IBI Scientific IB47010 GelPCR DNA Fragment Extraction Sample Kit for 4 Preparations. Order yours today and experience the convenience and effectiveness of this high-quality product.

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Customer Questions & Answers

  • Question: What is the IB47010 GelPCR DNA Fragment Extraction Sample Kit used for?

    Answer: The IB47010 GelPCR DNA Fragment Extraction Sample Kit is designed for the purification of DNA fragments from agarose gel after gel electrophoresis. This kit helps researchers obtain high-quality DNA suitable for downstream applications such as cloning, sequencing, and PCR. By efficiently extracting DNA from gel, it ensures minimal loss of DNA and retains the integrity of the fragments. This kit is especially beneficial in molecular biology labs where precise DNA manipulation is crucial, making it an essential tool for genetic analysis and research.
  • Question: How many preparations can I make with the IB47010 kit?

    Answer: The IB47010 GelPCR DNA Fragment Extraction Sample Kit is specifically designed to facilitate up to four separate DNA fragment preparations. Each preparation allows for the extraction of DNA from gel plugs, making the kit ideal for small-scale experiments or projects. This is particularly useful for labs that may not require large quantities of DNA or need to conduct multiple experiments concurrently. The kit's efficiency helps ensure that each extraction maintains high yield and purity, critical for subsequent analyses.
  • Question: Is the extraction process time-consuming?

    Answer: The extraction process using the IB47010 GelPCR DNA Fragment Extraction Sample Kit is designed to be quick and efficient, typically taking about 30 minutes from start to finish. The kit includes all necessary reagents and protocols, streamlining the workflow. Quick extraction is especially crucial in research scenarios where time-sensitive results are necessary, such as during real-time experiments or when working with multiple samples. This efficiency allows scientists to focus more on analysis and less on preparation.
  • Question: Can this kit be used for different sizes of DNA fragments?

    Answer: Yes, the IB47010 GelPCR DNA Fragment Extraction Sample Kit is versatile and can be used to extract DNA fragments of various sizes. It is particularly effective for fragments ranging from 50 bp to 10 kb, catering to common applications in molecular biology. The inclusion of specialized buffers allows for selective recovery of desired fragment sizes, making it suitable for various research needs including cloning and sequencing. This flexibility makes the kit a great asset in labs dealing with diverse projects.
  • Question: What additional equipment or materials do I need to use this kit?

    Answer: To utilize the IB47010 GelPCR DNA Fragment Extraction Sample Kit effectively, you will need standard lab equipment such as a gel electrophoresis setup, a centrifuge, and micropipettes. Additionally, agarose gel and a DNA ladder are often used during gel electrophoresis to visualize DNA fragments. Having these essentials at hand ensures a smooth workflow while using the kit, thereby enhancing your laboratory's capabilities in DNA analysis and manipulation.
  • Question: What types of samples can I extract DNA from using this kit?

    Answer: The IB47010 GelPCR DNA Fragment Extraction Sample Kit is designed primarily for extracting DNA from agarose gel following gel electrophoresis. It can effectively purify DNA fragments obtained from PCR amplifications, restriction digests, and other applications leading to agarose gel electrophoresis. This specificity for gel-purified samples makes the kit particularly valuable for researchers focusing on genetic engineering, cloning, or any project requiring high-quality DNA samples for subsequent analysis.
  • Question: Is the DNA extracted using this kit suitable for sequencing?

    Answer: Yes, the DNA extracted using the IB47010 GelPCR DNA Fragment Extraction Sample Kit is highly suitable for sequencing applications. The kit ensures high yield and purity of the DNA, which are critical factors for successful sequencing. Purified DNA free from contaminants will lead to more accurate sequencing results, making this an essential tool for labs involved in genomic research or any project requiring precise DNA characterizations such as next-generation sequencing.
  • Question: How does the kit ensure the purity of the DNA fragments?

    Answer: The IB47010 GelPCR DNA Fragment Extraction Sample Kit employs a specialized purification process that includes a series of washing steps to remove proteins, agarose gel residues, and other contaminants. This meticulous process ensures that only high-quality DNA is collected for use in further applications. Pure DNA is crucial in molecular research, where contaminants can interfere with reactions or analyses, thus necessitating the importance of this kit in obtaining the finest DNA quality.
  • Question: Are the reagents in the kit ready for use?

    Answer: Yes, the reagents included in the IB47010 GelPCR DNA Fragment Extraction Sample Kit are pre-formulated and ready to use, streamlining your workflow. This eliminates the need for time-consuming preparation of reagents, allowing researchers to focus on their experiments rather than preparation. Ready-to-use reagents enhance reproducibility and consistency across experiments, making it an advantageous feature for labs looking to maintain high standards in their research.
  • Question: Where can I buy the IB47010 GelPCR DNA Fragment Extraction Sample Kit in Sierra Leone?

    Answer: You can buy the IB47010 GelPCR DNA Fragment Extraction Sample Kit from Ubuy. Ubuy offers a selection of scientific products including this DNA extraction kit, ensuring easy access for researchers and labs in Sierra Leone. With Ubuy, you can benefit from a reliable online shopping experience tailored for professionals seeking quality lab supplies.

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